Glossarium

Capillary gel electrophoresis is method of PCR products separation in electric field inside a capillary pre filled with polymer. When high voltage is applied, the fluorescently labeled PCR-products and other components start to pass move inside the capillary at the speed depending on their electric charge and mass and, therefore, arrive to the detection area at different times. The detected PCR-products are displayed as a sequence of fluorescent peaks. Therefore, migration time is the key quality characteristic to the molecule, whereas peak heights and areas comprise quantitative parameters of the sample.

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