A reagent kit
for detection and quantification of mRNAs produced by
chimeric CBFB-MYH11 gene and reference ABL gene in clinical material by polymerase chain reaction (PCR) with
real-time hybridization-fluorescence detection.
The reagent
kit can be used to reveal AML associated with CBFB-MYH11 chromosomal
rearrangement, to confirm the AML diagnosis, and to monitor the efficiency of
therapy, i.e., MRD
evaluation.
The reagent kit
is designed to conduct a study in a quantitative format for 24 clinical samples
in duplicate (132 PCR reactions, including controls).
Additional
information about the molecular marker
Chromosome 16
inversion inv(16)(p13q22) occurs in about 8-9% of primary acute myeloid
leukemia (AML), and results in fusion of the CBFb gene, which encodes part of the CBF transcription factor and MYH11 gene encoding the heavy
chain of myosin smooth muscle fibers. A total of 10 transcript
variants of the CBFB-MYH11 gene were found: transcript A is detected in more
than 85% of inv(16)-positive patients, types D and E are detected in about 5%
of patients, the remaining variants occur rarely (Figure 1).
Figure 1. Arrangement of CBFB-MYH11 A, D, and E chimeric transcripts. Binding sites for primers and probes for real-time PCR are marked. The positions of primers and probes are indicated relative to the 5' end of the nucleotide sequences of normal transcripts.
AML patients with
chromosome 16 inversion usually have a favorable prognosis, with more than 50%
of patients achieving complete remission. Accordingly, CBFB-MYH11 translocation
is an important target in the diagnosis and monitoring of minimal residual
disease (MRD). When compared with
standard methods (karyotyping, FISH), usage of real-time
PCR (Real-Time Quantitative PCR, RQ-PCR) which measures CBFB-MYH11
expression levels provides significantly higher sensitivity when
assessing minimal residual disease (MRD), revealing one malignant
cell among 50,000 normal ones.
More detailed information on diagnostic approaches, frequency of the study and prognostic value of this testing in leukemias are available on the website of the International Network European Leukemia Net (http://www.leukemia-net.org).
Ordering information:
CBFB-MYH11 A RQ Kit, 24 tests Cat.No IG-RQ-3-24
CBFB-MYH11 D RQ Kit, 24 tests Cat.No IG-RQ-4-24
Additiional reagents:
Blood RNA stabilizer medium Cat.No IG-RSB-100
TriZ Reagent Kit Cat.No IG-TRK-100
ReverZyme Kit Cat.No IG-RT-1
Erythrocyte Lysis Solution Cat.No IG-TRL-100
Price: on request
Reagents are for research use only (RUO)